POR QUÉ DEBERÍA DUDARSE QUE EL COVID-19 SEA UN SUCESO NATURAL (12-07-2020+12-12-2022)

POR QUÉ DEBERÍA DUDARSE QUE EL COVID-19 SEA UN SUCESO NATURAL

 

 

 

Forensic Analysis of Novel SARS2r-CoV Identified in Game Animal Datasets in China Shows Evolutionary Relationship to Pangolin GX CoV Clade and Apparent Genetic Experimentation

by

Adrian Jones

¹,

Steven E. Massey

^2,*,

Daoyu Zhang

³,

Yuri Deigin

⁴ and

Steven C. Quay

⁵

¹

Independent Bioinformatics Researcher, Melbourne, VIC 3000, Australia

²

Biology Department, University of Puerto Rico-Rio Piedras, San Juan, PR 00925, USA

³

Independent Genetics Researcher, Sydney, NSW 2120, Australia

⁴

Youthereum Genetics Inc., Toronto, ON L4J 8G9, Canada

⁵

Atossa Therapeutics, Inc., Seattle, WA 98104, USA

^*

Author to whom correspondence should be addressed.

Appl. Microbiol. 2022, 2(4), 882-904; https://doi.org/10.3390/applmicrobiol2040068

Received: 10 October 2022 / Revised: 31 October 2022 / Accepted: 1 November 2022 / Published: 7 November 2022

(This article belongs to the Special Issue Microbiome in Ecosystem 2.0)

 

Abstract

Pangolins are the only animals other than bats proposed to have been infected with SARS-CoV-2 related coronaviruses (SARS2r-CoVs) prior to the COVID-19 pandemic. Here, we examine the novel SARS2r-CoV we previously identified in game animal metatranscriptomic datasets sequenced by the Nanjing Agricultural University in 2022, and find that sections of the partial genome phylogenetically group with Guangxi pangolin CoVs (GX PCoVs), while the full RdRp sequence groups with bat-SL-CoVZC45. While the novel SARS2r-CoV is found in 6 pangolin datasets, it is also found in 10 additional NGS datasets from 5 separate mammalian species and is likely related to contamination by a laboratory researched virus. Absence of bat mitochondrial sequences from the datasets, the fragmentary nature of the virus sequence and the presence of a partial sequence of a cloning vector attached to a SARS2r-CoV read suggests that it has been cloned. We find that NGS datasets containing the novel SARS2r-CoV are contaminated with significant Homo sapiens genetic material, and numerous viruses not associated with the host animals sampled. We further identify the dominant human haplogroup of the contaminating H. sapiens genetic material to be F1c1a1, which is of East Asian provenance. The association of this novel SARS2r-CoV with both bat CoV and the GX PCoV clades is an important step towards identifying the origin of the GX PCoVs.

 

Keywords: 

 

pangolin; coronavirus; SARS-CoV-2; game animal; forensic; metagenomics; Guangxi

 

 https://www.mdpi.com/1930674